Isolated Adult Cardiac Myocytes: Structure, Function and Metabolism -

Isolated Adult CardiomyocytesStructure and Metabolism.

Nov 01, 1985 · Isolated Adult Cardiac Myocytes: Structure, Function and Metabolism [Spieckermann, P. G.] on. FREE shipping on qualifying offers. Isolated Adult Cardiac Myocytes: Structure, Function and Metabolism. 6. Biochemistry of Sarcolemma of Isolated Cardiomyocytes. 7. Adrenergic, Cholinergic, and Other Hormone Receptors on Cardiac Myocytes. ENERGY METABOLISM. 8. Oxygen Delivery and Oxidative Phosphorylation in Isolated Adult Ventricular Myocytes. 9. Metabolism of Purines by Adult Cardiomyocytes. 10. Substrate Metabolism of Isolated Cardiomyocytes. Jul 17, 2019 · First published in 1989, written with contributions from experts who have critically evaluated the state of the art and the prospects of isolated adults cardiac. Isolated heart cells as a model of the myocardium --The isolation and characterization of calcium-tolerant myocytes --Functional alterations of the sarcolemma in Ca²-free perfused hearts --Intracellular Ca² shifts during the Ca²⁺ paradox --Surface structures of the heart cell and their role in contractile control --Ultrastructure of the.

Get this from a library! ISOLATED ADULT CARDIOMYOCYTES: structure and metabolism. [Hans Michael Piper; Gerrit Isenberg] -- First published in 1989, written with contributions from experts who have critically evaluated the state of the art and the prospects of isolated adults cardiac myocytes in cardiological research. Jul 06, 2018 · Myocyte formation in the adult non-mammalian heart. Studies in amphibians and teleost fish such as the zebrafish were among the first to demonstrate experimentally that the postnatal heart could generate new myocytes 4, 5.In adult zebrafish, surgical resection of up to 20% of the ventricle resulted in the formation of a transient fibrin-rich clot, followed by a robust increase in cycling. Nov 01, 2002 · The myocytes are extraordinary cells. They are immortal and contract for a lifetime, supporting the peripheral circulation. In order to do so, they have a unique ultrastructure and unique biochemical machinery that allows them to produce enough adenosine triphosphate to support the contraction.

These cardiac myocytes were studied for their contractile function with video-edge detection and calcium metabolism with indo-1 fluorescence spectroscopy after exposure to these uremic sera. These uremic sera caused increases in myocyte fractional shortening P < 0.01 as well as an increase in the time constant of relengthening P < 0.01. 1. The effects of glucose, pyruvate and lactate on basal metabolism and on contraction-related energy expenditure of thin trabeculae isolated from guinea-pig heart were studied using a microcalorimetric technique. 2. Resting heat rates of cardiac ventricular. Capacity and Flexibility of Substrate Metabolism for ATP Production. The heart is capable of using all classes of energy substrates, including carbohydrates, lipids, amino acids, and ketone bodies, for ATP production in the mitochondrion Figure 1, for details see reviews. 1 – 3 Mitochondria occupy one third of the cell volume in cardiac myocytes, making them the cell type with the highest.

Isolated Adult Cardiac Myocytes: Structure, Function and Metabolism

By now the basic features of the energy metabolism of isolated cardiomyocytes have been described. Unstimulated cardiomyocytes are quiescent and exhibit the characteristics of cardiac metabolism at basal energie demand. Their energetic demand, however, can be stimulated to the maximal rates of the physiological range. Effects of chronic administration of clenbuterol on function and metabolism of adult rat cardiac muscle Gopal K. R. Soppa,1 Ryszard T. Smolenski,1 Najma Latif,1 Ada H. Y. Yuen,1 Aalya Malik,1 Joanna Karbowska,2 Zdzislaw Kochan,2 Cesare M. N. Terracciano,1 and Magdi H. Yacoub1 1Imperial College London, National Heart and Lung Institute, Harefield Heart Science Centre.

Sex differences in baseline cardiac function & disease. Cardiovascular disease CVD is the leading cause of death of American men and women. 1 Even though CVD causes 1/3 of women’s deaths each year, women have traditionally been excluded from clinical trials and female animals have been utilized less or sex was not reported in basic research studies. 1, 2 Until recently, consideration of. We also confirmed poor Ca 2-handling in cardiac myocytes isolated from adult C-RAMP2 −/−. We found that the altered expression of BNP after RAMP2 deletion was preceded by altered expression of the transcriptional coactivator PGC-1α, which governs mitochondrial biology through broad regulation of genes in both the nuclear and mitochondrial. Metabolism in organs other than the liver and kidneys may play a significant role in how a specific organ responds to chemicals. The heart has metabolic capability for energy production and homeostasis. This homeostatic machinery can also process xenobiotics. Cardiac metabolism includes the expression of numerous organic anion transporters, organic cation transporters, organic carnitine. Jan 22, 2013 · Myocytes were isolated with the use of a Percoll GE Healthcare gradient and plated on 35-mm glass-bottom culture dishes. Adult rat ventricular myocytes ARVM were enzymatically isolated from the heart of 3–4-mo-old male Fisher rats as described previously Goldhaber et al., 1989.

New Myocyte Formation in the Adult HeartEndogenous.

Methods and Results We studied serial changes in global left ventricular LV structure and function in infarcted 1, 2, 4, and 6 weeks after myocardial infarction and sham-operated rat hearts and correlated them with structural and functional changes in myocytes isolated from the remote LV myocardium in the same hearts. Rats with myocardial. Uptake and metabolism of palmitate by isolated cardiac myocytes from adult rats: Involvement of sarcolemmal proteins Article PDF Available in Journal of Lipid Research 384:745-58 · May 1997.

tumor necrosis factor-α TNF-α is a pleiotropic peptide that is elevated in the sera and myocardium of patients with heart failure HF.Its importance in the pathophysiology of left ventricular LV dysfunction was first recognized in studies demonstrating acute negative inotropic effects on single cardiac myocytes and isolated heart preparations 1, 14, 16, 26, 61. Ventricular myocytes were isolated from adult Wistar rats. Choline uptake and metabolism were examined, using [ methyl - 3 H]choline. Choline metabolism to phosphatidylcholine PC, an important phospholipid for cellular structure and function, was reduced by mild hypoxia while choline uptake. Myocytes have been isolated from adult rat hearts since 1969. The early preparations exhibited the Ca 2 paradox. Over the ensuing years, numerous groups have reported the isolation of Ca 2 tolerant cardiac myocytes. In the present review, detailed comparisons have been made of the yields, viability, and relative Ca 2 tolerance of these different myocyte preparations.

Uptake and metabolism of palmitate by isolated cardiac myocytes from adult rats: involvement of sarcolemmal proteins. J Lipid Res. 1997; 38:745–758. Medline Google Scholar; 3 Sorrentino D, Stump D, Potter BJ, Robinson RB, White R, Kiang CL, Berk PD. Oleate uptake by cardiac myocytes is carrier mediated and involves a 40-kD plasma membrane. Jun 01, 2020 · To investigate protein expression profiles, cardiac cells were isolated from rat hearts and analyzed by high-resolution mass spectrometry. Cardiomyocytes were isolated by collagenase treatment using a modified Langendorff method, where oxygenated solutions at 37 °C were perfused through the heart [, ].Cardiomyocytes were separated from non-myocyte cells by size exclusion using a cell. Nov 01, 2019 · In adult cardiac myocytes, the precise matching of energy supply with demand are critical in maintaining cardiac function at different workloads and substrate supply. Heterogeneity of mitochondrial energetics can cause complex dynamic behavior to deal with these changes in cellular workload or environmental conditions [ 6 ].

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